The potential applications of protein G include practically all of the current and projected applications of protein A . Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Compared to protein A, protein G binds more strongly to polyclonal IgG, for example, from cow, sheep and horse. Furthermore, unlike protein A, protein G binds polyclonal rat IgG, human IgG3 and mouse IgG1.
Filter within Protein G Clear Package Type
Column (2) Resin (3)
Clear pH Stability
2 - 10 (short term) (5) 2 - 14 (short term) (0) 3 - 12 (long term) (0) 3 - 9 (long term) (5)
Clear Particle Size
100 - 350 μm (0) 150 - 350 µm (1) 20 - 50 μm (2) 50 - 150 μm (2)
Clear Operational Pressure
(0 - 6000 psi) (0) 0 - 40 MPa (0) Up to 3 bar (5)
Clear Pressure-flow Property
>2000 cm/h (0) >500 cm/h (0) 0.001 - 10.000 ml/min (analytical) (0) 0.001 - 4.000 ml/min (micro) (0) 0.001 – 2.000 ml/min (micro) (0) 0.01 to 10 ml/min (analytical) (0) 0.01 to 4.00 ml/min (micro) (0) 0.1 - 40.000 ml/min (semi-preparative) (0) 0.2 - 3.0 ml/min (analytical) (0) 0.2 - 3.0 ml/min (micro) (0) 0.2 to 40 ml/min (preparative) (0) 1.0 - 50.0 ml/min (preparative) (0) 100 cm/hr is recommended (0) 30 - 150 cm/hr is recommended (0) 50 - 300 cm/hr is recommended (5)
Clear Binding Capacity
>100 mg/ml BSA (0) >100 mg/ml lysozyme (0) >110 mg lysozyme / ml (0) >130 mg BSA / ml (0) >130 mg lysozyme / ml (0) >15 mg human IgG/mL (5) >150 mg/ml BSA (0) >150 mg/ml lysozyme (0) >20 mg/ml hIgG (0) >30 mg human IgG/mL (0) >40 mg/ml (0) >60 mg/ml hIgG (0)
Clear Total Ionic Capacity
>15 µmol/ml (0) 0.08 - 0.13 mmol/ml (0) 0.09 - 0.15 mmol/ml (0) 0.1 - 0.2 mmol/ml (0) 12 - 25 µmol/ml (0) 30 - 50 mmol/ml (0)
Clear Functional Group
Carboxy-methyl weak cation (0) Diethyl-aminoethyl weak anion (0) Nitrilotriacetic acid (NTA) (0) Quaternary ammonium strong anion (0) Sulfo strong cation (0)
Clear Matrix
Cross-linked 6% agarose (0) Cross-linked 6% beaded agarose (0) Dextran and agarose composite (0) Highly cross-linked 6% agarose (0) Highly cross-linked agarose (0) Highly cross-linked agarose and dextran (0) Highly cross-linked agarose beads (5) Highly cross-linked agarose grafted with dextran chain (0)
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