Protein A SepFast is a family of affinity chromatography media for the purification of immunoglobulins. Its purification power has been well documented in various antibody purification applications, such as isolation and purification of classes, subclasses and fragments of immunoglobulins from biological fluids and from cell culture media.
Filter within Protein A Clear Package Type
Column (2) Resin (2)
Clear pH Stability
2 - 10 (short term) (4) 2 - 14 (short term) (0) 3 - 12 (long term) (0) 3 - 9 (long term) (4)
Clear Particle Size
100 - 350 μm (0) 150 - 350 µm (1) 20 - 50 μm (2) 50 - 150 μm (1)
Clear Operational Pressure
(0 - 6000 psi) (0) 0 - 40 MPa (0) Up to 3 bar (4)
Clear Pressure-flow Property
>2000 cm/h (0) >500 cm/h (0) 0.001 - 10.000 ml/min (analytical) (0) 0.001 - 4.000 ml/min (micro) (0) 0.001 – 2.000 ml/min (micro) (0) 0.01 to 10 ml/min (analytical) (0) 0.01 to 4.00 ml/min (micro) (0) 0.1 - 40.000 ml/min (semi-preparative) (0) 0.2 - 3.0 ml/min (analytical) (0) 0.2 - 3.0 ml/min (micro) (0) 0.2 to 40 ml/min (preparative) (0) 1.0 - 50.0 ml/min (preparative) (0) 100 cm/hr is recommended (0) 30 - 150 cm/hr is recommended (0) 50 - 300 cm/hr is recommended (4)
Clear Binding Capacity
>100 mg/ml BSA (0) >100 mg/ml lysozyme (0) >110 mg lysozyme / ml (0) >130 mg BSA / ml (0) >130 mg lysozyme / ml (0) >15 mg human IgG/mL (0) >150 mg/ml BSA (0) >150 mg/ml lysozyme (0) >20 mg/ml hIgG (0) >30 mg human IgG/mL (4) >40 mg/ml (0) >60 mg/ml hIgG (0)
Clear Total Ionic Capacity
>15 µmol/ml (0) 0.08 - 0.13 mmol/ml (0) 0.09 - 0.15 mmol/ml (0) 0.1 - 0.2 mmol/ml (0) 12 - 25 µmol/ml (0) 30 - 50 mmol/ml (0)
Clear Functional Group
Carboxy-methyl weak cation (0) Diethyl-aminoethyl weak anion (0) Nitrilotriacetic acid (NTA) (0) Quaternary ammonium strong anion (0) Sulfo strong cation (0)
Clear Matrix
Cross-linked 6% agarose (0) Cross-linked 6% beaded agarose (0) Dextran and agarose composite (0) Highly cross-linked 6% agarose (0) Highly cross-linked agarose (0) Highly cross-linked agarose and dextran (0) Highly cross-linked agarose beads (4) Highly cross-linked agarose grafted with dextran chain (0)
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