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The Protein G SepFast HiSep column has a particle size of 50 – 150 µm and is good for most applications from small scale to large scale purification requirements (i.e. low column pressure).
Description
The Protein G SepFast HiSep column has a particle size of 50 – 150 µm, good for most applications from small scale to large scale purification requirements (i.e. low column pressure).
Protein G SepFast is a family of affinity chromatography media for the purification of immunoglobulins and binds to the Fc region of IgG from a variety of mammalian species. Protein G SepFast may be used to isolate and purify classes, subclasses and fragments of immunoglobins from any biological fluid or cell culture medium. Protein G SepFast is extremely useful for isolation of immune complexes.
The potential applications of protein G include practically all of the current and projected applications of protein A. Protein G and protein A, however, have different IgG binding specificities, dependent on the origin of the IgG. Compared to protein A, protein G binds more strongly to polyclonal IgG, for example, from cow, sheep and horse. Furthermore, unlike protein A, protein G binds polyclonal rat IgG, human IgG3 and mouse IgG1.
Protein G SepFast is developed and supported for production scale chromatography. We offer both loose medium and pre-packed ready-to-use disposable columns.
Additional information
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Class | |
| Volume | 1 x 1 ml, 1 x 5 ml, 5 x 1 ml, 5 x 5 ml |
| Matrix | Highly cross-linked agarose beads |
| Particle size | |
| Operational pressure | Up to 3 bar |
| Pressure-flow property | 50 – 300 cm/hr is recommended |
| Binding capacity | >15 mg human IgG/mL |
| Chemical stability | Stable in most commonly used buffers, 6 M guanidine-HCl; 70% ethanol, 8 M urea, pH 10.5; 0.1 M Glycine-NaOH, pH 11 |
| pH stability | 2 – 10 (short term), 3 – 9 (long term) |
| Storage | 20% ethanol |
