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IMAC SepFast has a particle size of 50 – 150 µm and is designed for the purification of histidine-tagged proteins in batch, gravity flow, packed bed, or 96-well microplate operations.
Description
IMAC SepFast has a particle size of 50 – 150 µm and is designed for the purification of histidine-tagged proteins in batch, gravity flow, packed bed, or 96-well microplate operations.
Key benefits:
- Low cost due to the novel manufacturing processes
- High binding capacity (could be over 40 mg/ml)
- Fast and easy protein purification from unclarified or clarified samples
- Choice of three pre-charged metal ions
- Very low metal leakage
- Full protein purification manual supplied
Its carefully controlled pore structure allows fast access of the target protein to the immobilised metal ligand. Also, its high mechanical strength permits liquid to pass through a column at excellent flow rates. Clarified or unclarified cell lysates (for intracellular proteins) or culture broths (for extracellular proteins) can be directly processed with IMAC SepFast. All these translate into high process flexibility and higher protein yield at a shortened purification time.
IMAC SepFast or its three metal charged formats i.e. Ni SepFast, Co SepFast and Zn SepFast, are highly stable and compatible with a wide range of chemicals (e.g. detergents, denaturing reagents and reducing reagents etc) commonly used in protein purification processes, which means that more flexible operations can be developed for the best performance.
Additional information
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Class | |
| Volume | 10 ml, 25 ml, 100 ml, 1 litre |
| Particle size | |
| Matrix | Highly cross-linked 6% agarose |
| Total ionic capacity | 12 – 25 µmol/ml |
| Binding capacity | >40 mg/ml |
| Chemical stability | Stable in 0.01M HCl, 0.1M NaOH and 0.2M acetic acid tested for one week, Stable in 0.1M HCl and 1% SDS tested for 30 mins, Stable in 0.5 M NaOH and 30% acetic acid tested for overnight |
| pH stability | 2 – 14 (short term), 3 – 12 (long term) |
| Storage | 20% ethanol |
