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The HiSep SP SepFast HighRes Plus column belongs to a group of strong cation ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution and high binding capacity purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.
Description
The HiSep SP SepFast HighRes Plus column belongs to a group of strong cation ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution and high binding capacity purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.
The core advantages are:
- High binding capacity
- High resolution
- High sample loading capacity at high flow
- High productivity
IEX SepFast HighRes Plus is made of a composite of agarose and dextran to give increased dynamic binding capacities. The base matrix is highly cross-linked. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.
Additional information
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Class | |
| Volume | 5 x 1 ml, 5 x 5 ml |
| Matrix | Highly cross-linked agarose grafted with dextran chain |
| Functional group | |
| Total ionic capacity | 0.09 – 0.15 mmol/ml |
| Particle size | |
| Binding capacity | >60 mg/ml hIgG |
| Pressure-flow property | 30 – 150 cm/hr is recommended |
| Operational pressure | Up to 3 bar |
| pH stability | 2 – 14 (short term), 3 – 12 (long term) |
| Working temperature | 4 ºC – 30 ºC |
| Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol |
| Avoid | Cationic detergents, Oxidizing agents |
| Storage | 20% ethanol + 0.2M sodium acetate |
