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The HiSep SP SepFast 6HF Plus column belongs to a group of strong cation exchange media, designed for the general purification of biological molecules where high binding capacity or faster binding kinetics is desirable.
Description
The HiSep SP SepFast 6HF Plus column belongs to a group of strong cation exchange media.
The above ion-exchange chromatography (IEX) media is designed for the general purification of biological molecules where high binding capacity or faster binding kinetics is desirable.
The base matrix is made of highly cross-linked 6% agarose and dextran. It is very stable to most of the chemical conditions experienced in the bioprocessing industry. The IEX SepFast 6HF Plus could be a good alternative to the IEX Sepharose XL, with the benefit of reduced cost. We provide the regulatory support files of individual resins for those cGMP applications.
The core advantages to large-scale biomanufacturings are:
- Much increased dynamic binding capacity
- Fast binding kinetics
- Affordable cost
Additional information
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Class | |
| Volume | 5 x 1 ml, 5 x 5 ml |
| Matrix | Dextran and agarose composite |
| Functional group | |
| Total ionic capacity | 0.09 – 0.15 mmol/ml |
| Binding capacity | >130 mg lysozyme / ml |
| Particle size | |
| Pressure-flow property | >500 cm/h |
| Operational pressure | Up to 3 bar |
| pH stability | 2 – 14 (short term), 3 – 12 (long term) |
| Working temperature | 4 ºC – 30 ºC |
| Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol |
| Avoid | Cationic detergents, Oxidizing agents |
| Storage | 20% ethanol + 0.2M sodium acetate |
