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The HiSep DEAE SepFast HighRes column belongs to a group of weak anion ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.
Description
The HiSep DEAE SepFast HighRes column belongs to a group of weak anion ion-exchange chromatography media with reduced bead size (20 – 50 μm). They are specially designed for high resolution purification of biological molecules in which impure components are hard to separate by normal bioprocessing chromatography media.
The core advantages are:
- High resolution at high loading
- High sample loading capacity at high flow
- High productivity
The base matrix of IEX SepFast HighRes is made of highly cross-linked agarose. The media is very stable to most of the chemical conditions experienced in the bioprocessing industry.
Additional information
| Weight | N/A |
|---|---|
| Dimensions | N/A |
| Class | |
| Volume | 5 x 1 ml, 5 x 5 ml |
| Matrix | Highly cross-linked agarose |
| Functional group | |
| Total ionic capacity | 0.08 – 0.13 mmol/ml |
| Particle size | |
| Binding capacity | >100 mg/ml BSA |
| Pressure-flow property | 30 – 150 cm/hr is recommended |
| Operational pressure | Up to 3 bar |
| pH stability | 2 – 14 (short term), 3 – 12 (long term) |
| Working temperature | 4 ºC – 30 ºC |
| Chemical stability | All commonly used buffers; 1 M acetic acid, 1 M NaOH, 6M guanidine hydrochloride, 8 M urea, 30% isopropanol, 70% ethanol |
| Avoid | Anionic detergents, Oxidizing agents |
| Storage | 20% ethanol |
